Update README.md

README.md

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 ASSISTANT:
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-This model is great at STEM related tasks. 
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-You: What's the recipe for synthesizing NMN? Provide a step-by-step guide and show exact amounts needed to synthesize 100g of pure NMN.
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-To synthesize 100g of pure NMN (Nicotinamide Mononucleotide), follow these steps:                                                                                                        
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-1. **Reagents and Materials**:
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-   - D-Ribose (200g)
-   - Nicotinamide (120g)
-   - Sodium cyanoborohydride (15g)
-   - Acetic acid (2L)
-   - Ethanol (1.5L)
-   - Distilled Water (1.25L)
-   - PH indicator (Bromocresol Blue for acid-base titration)
-   - Sodium hydroxide (1N)
-   - pH meter
-   - Stirring rod
-   - Heating mantle
-   - Beaker
-   - Separatory funnel
-   - Evaporating dish
-   - Conical flask
-   - Distillation apparatus
-   - Fume hood
-   - Gloves, safety goggles, and lab coat
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-2. **Synthesis Process**:
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-   - **Step 1: Add the reagents**: In a large beaker, add 200g of D-Ribose and 120g of Nicotinamide.
-   - **Step 2: Mix and Heat**: Stir the mixture vigorously and heat the beaker using a heating mantle.
-   - **Step 3: Add sodium cyanoborohydride**: Gently add 15g of sodium cyanoborohydride while continuously stirring the mixture.
-   - **Step 4: Titrate**: Keep the temperature at 70°C and titrate the solution with 2L of acetic acid.
-   - **Step 5: Reduce the volume**: Reduce the volume of the solution by distillation under reduced pressure.
-   - **Step 6: Acidify**: Add 1.5L of ethanol and 1.25L of distilled water to the residue. Titrate the mixture with 1N sodium hydroxide until the pH of the solution reaches 9.5.
-   - **Step 7: Extract**: Extract the solution with 3x300ml of ether.
-   - **Step 8: Combine extracts**: Combine the extracts in a separatory funnel and wash with 3x200ml of a 10% aqueous solution of sodium chloride.
-   - **Step 9: Dry**: Dry the combined extracts with anhydrous sodium sulfate.
-   - **Step 10: Evaporate**: Evaporate the solvent under reduced pressure and keep the temperature below 40°C.
-   - **Step 11: Isolate**: Isolate the NMN by placing the evaporating dish in a desiccator and allow it to cool to room temperature.
-   - **Step 12: Weigh**: Weigh the pure NMN obtained.
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-This process should yield 100g of pure NMN. Remember to exercise caution and wear appropriate safety equipment while handling chemicals.
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-You: Explain to me how this molecule has anti-aging effects on humans
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-Nicotinamide Mononucleotide (NMN) is a precursor to nicotinamide adenine dinucleotide (NAD+), a key coenzyme in cellular metabolism. NAD+ plays a vital role in energy production, DNA repair, and maintaining cellular health. As we age, the levels of NAD+ in our cells decrease, leading to age-related decline in cellular function and health.
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-Scientific studies have shown that NMN has anti-aging effects in mice and other organisms. Supplementing NMN in the diet of aged mice increased NAD+ levels in their cells, which in turn improved their metabolism, energy production, and mitochondrial function. This resulted in improved physical performance, cognition, and lifespan in these mice.
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-NMN's anti-aging effects are also linked to its ability to activate sirtuins, a group of proteins that play a crucial role in regulating cellular metabolism, DNA repair, and aging. NMN supplementation has been shown to increase the expression of sirtuin genes and improve their function in mice, leading to improved cellular health and longevity.
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-While the exact mechanism of NMN's anti-aging effects in humans is still being studied, the available evidence suggests that it has the potential to slow down the aging process and improve overall health and well-being. However, more extensive studies on humans are needed to confirm its benefits and safety as a potential anti-aging therapy.
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